Tocopherol Alleviates Exacerbation of Colitis Due to Cadmium Exposure in Wistar Rats: A Biochemical and Histological Study
Published: 2024-09-03
Page: 165-174
Issue: 2024 - Volume 7 [Issue 1]
Ige Serah Funke
Department of Physiology, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
Ayinla Sussan Chinyere *
Department of Physiology, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
Adeniyi Iyanuoluwa Temilade
Department of Physiology, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
Yusuff Matthew Akorede
Department of Physiology, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
Adewumi Adeola Anuoluwa
Department of Physiology, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
Olaleke Faith Temitope
Department of Physiology, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
Hezekiah Oluwaseun Samuel
Department of Physiology, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
Ayodele Deborah Feranmi
Department of Physiology, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
Akangbe Barakat Omobolanle
Department of Physiology, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aims: This study investigated the effects of Tocopherol (vitamin E) on cadmium-exposed colitic rats.
Study Design: A randomized controlled experimental study involving 40 male Wistar rats (180 ± 20g). Group 1: Normal control; Group 2: Vitamin E-treated; Group 3: Colitis-induced; Group 4: Cadmium Chloride (CdCl2); Group 5: Colitis + CdCl2; Group 6: Colitis + vitamin E; Group 7: CdCl2 + vitamin E; Group 8: Colitis + CdCl2 + vitamin E.
Place and Duration of Study: Department of Physiology, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo state Nigeria, between January 2024 and May 2024.
Methodology: The experiment lasted 30 days. CdCl2 (50mg/kg b.w) was administered orally for 21 days, Colitis was induced on day 23 with 2 mL/200g of 6% acetic acid intra-rectally, while the animals were treated with vitamin E (100mg/kg b.w) for 7 days. Diarrhea score and body weight changes were assessed during the course of the experiment. Oxidative stress markers: Superoxidase dismutase (SOD) and Catalase (CAT); inflammatory markers: Myeloperoxidase (MPO) and Tumor Necrosis Factor alpha (TNF-α); Neutrophils and Lymphocyte counts and colon histology were also assessed. Data obtained was analyzed using one-way ANOVA, and Tukey’s post hoc test was used for comparison of inter-group differences. P < 0.05 was considered statistically significant.
Results: The study showed significant body weight decrease and increased diarrhea scores in cadmium-exposed and colitis + cadmium groups. SOD and CAT activities were significantly depleted, while MPO activity and TNF-α levels were markedly elevated compared to normal control and vitamin E-treated groups. Neutrophil count decreased while lymphocyte count increased in Cd-exposed and colitis + cadmium groups compared to the normal control. Histological evaluation revealed inflammation and epithelial erosion in the colitis + cadmium group, with observed healing in vitamin E-treated groups.
Conclusion: This study revealed vitamin E’s potential to mitigate oxidative stress and inflammation in colitis when heightened by cadmium toxicity. This findings provides new insights into the role of vitamin E in preventing and treating inflammatory conditions.
Keywords: Ulcerative colitis, vitamin E, cadmium chloride, oxidative stress